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To investigate the effect of Trypsin using a photographic …
- https://blablawriting.net/prime-investigate-effect-trypsin-using-photographic-film-essay
- A beaker was filled with water of about 400C. Each test tube was filled with 5cm3 of trypsin and 5cm3 of water with a buffer solution one pH 4 one pH 7 and the other pH 9. A piece of 1cm2 photographic film was dropped in. At this time the stop clock was started. Conclusion: Trypsin works best at pH 9. Temperature experiment:
To investigate the effect of Trypsin using a photographic film
- https://www.markedbyteachers.com/as-and-a-level/science/to-investigate-the-effect-of-trypsin-using-a-photographic-film.html
- The higher temperature gave the active site become more elastic to change shape to fit the substrate. My conclusions are that trypsin works best at an alkali pH and a temp of 710 C. Evaluating: In my experiment I think that we should of used a bigger range of temperatures because then we may have been able to see when the enzyme denatured.
Investigating effect of concentration on the activity of trypsin
- https://practicalbiology.org/bio-molecules/factors-affecting-enzyme-activity/investigating-effect-of-concentration-on-the-activity-of-trypsin
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A spectrophotometric determination of trypsin and
- https://www.sciencedirect.com/science/article/pii/0006300255902808
- The extinction coefficient used for chymotrypsin is 2.o75/cm/mg/mlla and that for trypsin 1.7I/cm/mg/m114. RESULTS The difference spectra of o.ool M solutions of L-tyrosine at various pH values with reference to a o.ooi M solution of L-tyrosine at pH 3.o7 are shown in the lower part of Fig. I. The effect of dissociable groups other than the ...
Biology Coursework: the Effect of Trypsin on Gelatine.
- https://www.studymode.com/essays/Biology-Coursework-The-Effect-Of-Trypsin-58766.html
- § Photographic film (b/w) § Syringe § Test tube rack § Ruler § Scissors § O.1% trypsin made in pH7 buffer solution § Mounted needle 2. I then decided on the range of results and temperatures I was to investigate. I decided to observe 20º, 30º, 40º, 50º, 60º, 70º and 80º. 3. Next, I label each test tube with the temperature to be ...
To investigate the effect of temperature on the rate of …
- https://blablawriting.net/prime-investigate-effect-temperature-rate-reaction-trypsin-enzymes-essay
- * Ensure the same amount of trypsin in used throughout the experiment. * Ensure the same size of photographic film is used throughout the experiment. * Carry out the experiment three times to ensure the results are valid and are reasonable. * Ensure the trypsin is acclimatised before the experiment. * Ensure the same water bath is used.
Trypsin Lab Report - 928 Words | Cram
- https://www.cram.com/essay/Trypsin-Experiment/PCARSZA4U
- Trypsin is a proteolytic enzyme, important for the digestion of proteins. Enzymes are biological catalysts for metabolic process in cells. A catalyst is a substance that changes the rate of a reaction. Enzymes act as catalysts for chemical reactions in the body. Enzymes are large proteins that are not consumed or changed in a chemical reaction.
Procedure - Nuffield Foundation
- https://www.nuffieldfoundation.org/sites/default/files/files/investigating-effect-of-concentration-on-the-activity-of-trypsin-ss-33%20(1).docx
- Investigating effect of concentration on the activity of trypsin. In this practical you will learn to: ... Cut pieces of photographic film to fit in the test tube as shown. (This may have been done for you.) ... Plot a suitable graph of the results. This could be a graph of time taken for the reaction to occur against temperature. Or, you can ...
Investigating The Rate Of Reaction Of Enzymes In The Clearing Of ...
- https://essaymania.com/50508/investigating-the-rate-of-reaction-of-enzymes-in-the-clearing-of-photographic-film
- The experiment must be a fair test or I will be getting a lot of anomalous results and there may not be a clear pattern in the results. To make the experiment a fair test I am going to make sure that volumes of trypsin and pH buffer are the same in each experiment and the size of the photographic film is the same.
pH effects on the reaction rates of Trypsin by Ehsaan …
- https://prezi.com/0f3kfkj8ha5l/ph-effects-on-the-reaction-rates-of-trypsin/
- Basic Method. Fill 6 test tubes with 2% trypsin (A-F) and a different pH buffer solution (4-11) (Experimental) Heat water bath to 38 degrees, place in test tubes, then place a wooden splint with film attached into each test tube. Start to …
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